fortiusbio产品代理
fortiusbio产品代理
后来重点转移到使用我们的 RNA 稳定性保持试剂处理滤纸卡上，以便在环境温度下保存流体样品 RNA。
RNASound™ ReadyPunched™ RNA 采样卡是经过专利化学处理的滤纸卡，它前所未有地在室温下长时间保持 RNA 的完整性；每张卡都有两个 5 毫米的穿孔圆盘，可以很容易地用移液器吸头拆下。最大 100uL 的流体样品可以滴在卡上或用棉签在卡上擦洗。干燥后，通过在 Card Elution Solution 中短暂摇动，可以轻松地从分离的圆盘上洗脱 RNA。加州公共卫生部将我们的卡与 FTA 卡进行了比较，最终选择了我们的卡来执行自 2014 年以来的例行西尼罗河病毒监视任务，他们最近公布了详细的比较结果；世界卫生组织 (WHO) 发表的观察结果表明，流感病毒 RNA 在卡上在环境温度下可保持稳定长达 4 周。
RNASound™ 血液 RNA 卡是一种较厚的滤纸卡，经过专有化学品处理，专门用于血液样本。每张卡上最多可以应用 200uL 的血液，并保持 RNA 的完整性，随后使用标准 RNA 提取方法提取 RNA。西雅图儿童研究所发表的结果表明，与基于基质的管产品相比，卡片中 HIV-1 RNA 的保存和回收效果更好。
LyseNow® 穿孔卡片是厚滤纸卡片，适用于所有样品基质，包括血液、RNA 和 DNA。每张卡都有七个穿孔的 3 毫米圆盘，便于用移液器吸头拆下。每张卡都有独特的条形码，便于样品和结果记录和跟踪。穿孔卡片的设计是一项未决的美国外观设计专利申请 (US 29578820) 的主题。
LyseNow® Strip Card 是一个塑料条，每个都有八个 5 毫米专有化学品处理过的滤纸盘。八个圆盘按照标准的 96 孔板孔间距排列，允许它们通过多道移液器立即转移到板行中的孔中。每个圆盘上装载多达 10uL 的流体样品并干燥，圆盘很容易用移液器吸头分离。该卡用于 RNA、质粒 DNA 或病原体 DNA 的高通量提取。针对不同的样品基质开发了不同的洗脱方案，感兴趣的目标是 RNA 或 DNA。条卡设计是待批的美国外观设计专利申请 (US 29578821) 的主题。
FortiusBio was registered in 2010, inspired by the discovery that sodium pyrophosphate, among several other chemicals, enhances RNA stability in guanidium based tissue lysates. The discovery was the subject of a PCT patent application of PCT/US2011/063189. We sell the proprietary RNA preserving reagent as additives to the standard guanidium based tissue lysis buffer for enhanced RNA stability during RNA extraction, we also market RNA preserving reagent enhahnced tissue lysis buffer as means to preserve sample RNA at ambient temperature during storage and transportation.
Focus was later shifted to treating filter paper card with our RNA stability preserving reagents for fluid sample RNA
preservation at ambient temperature.
RNASound™ ReadyPunched™ RNA Sampling card is filter paper card that is treated with the proprietary chemicals, it unprecedentedly preserves RNA integrity at ambient temperature for prolonged time period; Each card has two 5mm perforated discs that are easy to be detached with pipette tips. Fluid sample up to 100uL can be dropped on the card or be scrubbed on the card with swabs. After dry, RNA can be easily eluted from detached discs by brief shaking in Card Elution Solution. California Department of Public Health compared our card with FTA card and eventually selected our cards for their routine West Nile Virus surveillance task since 2014, they recently published details the comparison results; World Health Organization (WHO) published observation that influenza virus RNA stayed stable on the card at ambient temperature for up to 4 weeks.
RNASound™ Blood RNA card is a thicker filter paper card that is treated with the proprietary chemicals and used specifically for blood samples. Up to 200uL of blood can be applied on each card and integrity of RNA is preserved, RNA is later extracted with standard RNA extraction methods. Seattle Children's Research Institute published results of better HIV-1 RNA preservation and recovery from the card than from matrix based tube product.
LyseNow® Perforated Cards are thick filter paper card for all sample matrices, including blood, for both RNA and DNA. Each card has seven perforated 3 mm discs for easy detach with pipette tips. Each card has unique barcode for the ease of sample and result recording and tracking. The design of the perforated card is the subject of a pending US design patent application (US 29578820).
LyseNow® Strip Card is a plastic strip, each hosts eight 5 mm proprietary chemicals treated filter paper discs. Eight discs are arranged in accordance to the standard 96 well plate well spacing, allow them to be translocated at once to wells in the plate row with multi-channel pipette. Fluid samples up to 10uL is loaded on each disc and dried, discs are easily detached with pipette tips. The card is for high throughput extraction of RNA, plasmid DNA, or pathogen DNA. Different elution protocols were developed for different sample matrices and either RNA or DNA is the interested targets. The strip card design is the subject of a pending US design patent application (US 29578821).
