101bio 产品代理

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101bio 产品代理

Northern blot是一种分子生物学技术,最早于1977年开发,以其在基因表达、疾病诊断和病毒microRNA检测等方面的广泛应用而闻名。它是一种经典的方法,用于表征和定量样本基质中的特异性RNA基因。northern blot分析首先从组织或细胞中提取RNA,然后用变性琼脂糖凝胶进一步分离。分离的RNA通过毛细管力转移到尼龙膜上。然后将膜上的RNA与互补探针杂交进行检测,互补探针可以用生物素、地高辛结合物或放射性同位素标记。随着对辐射暴露的监管要求越来越严格,我们专门从事非放射性Northern Blot技术开发,以满足迫切的科学需求。请联系我们获取以下高效服务的报价:

RNA纯化与整合分析
绿色变性体系分离RNA凝胶
RNA膜转移
敏感探针标记
探针杂交
图像开发和数据分析
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Northern blot is a molecular biological technique first developed in 1977, known for its wide application on gene expression, disease diagnosis, and viral microRNAs detection, etc. It is a classical methodology to characterize and quantitate specific RNA genes in the sample matrix. The northern blot analysis first starts from RNA extraction from tissues or cells, which are further separated by denaturing agarose gels. The separated RNAs are transferred to a nylon membrane with capillary forces. RNA on the membrane is then hybridized with complementary probes for detection, which could be labeled with biotin, Digoxigenin conjugated or radioactive isotope-labeled. With increasingly strict regulatory requirements on radiation exposure, we are specialized in non-radioactive Northern Blot technical development to meet urgent scientific needs. Contact us to get a quote for the following efficient services:

RNA purification and integration analysis

RNA gel separation with greener denaturing system

RNA membrane transfer

Sensitive probe labeling

Probe hybridization

Image development and data analysis


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