silantes产品代理
silantes产品代理
Silantes 使用一种新的生物技术方法生产生物分子，例如用稳定同位素2 H、13 C 和15 N标记的氨基酸、核苷酸、肽、蛋白质和 DNA 。
前一项技术是由马克斯普朗克生物化学研究所的 Hermann Heumann 博士于 1998 年开发的。
该技术基于化学自养细菌可以以类似于绿色植物的途径代谢无机碳的事实。化学自养细菌结合了绿色植物能够同化CO 2 的优势和细菌更高产并且还能够表达异源蛋白质的优势。
标记的化学自养细菌细胞被用作生产稳定同位素标记生物分子的工厂。这项开创性技术提供了用稳定同位素标记的组分的出色同质性，特别是在三重标记（2 H、 13 C 和 15 N）生物分子领域。
Silantes Amino Acids
Silantes in-vivo SILAC SILAM
Silantes Dry Biomass
Silantes NMR Standards
Silantes Nucleotides
Silantes Cell Growth Media
Silantes in-vitro SILAC
Stable isotopes in NMR-spectroscopy
„Nuclear magnetic resonance “(NMR)-spectroscopy is used for 3D structure and dynamic clarifications. For this purpose, the molecule of interest gets labelled with the nuclear spin active stable isotopes 13C and 15N. Silantes focusses on NMR analyses of nucleic acids (RNA, DNA) and proteins. For these biomolecules, we produce isotopically labelled components, such as
Nucleosides and Nucleotides (go to the Webshop)
Phosphoramidites (go to the Webshop)
Custom oligo nucleotides (go to the Webshop)
Cell growth media and M9 reagents (go to the Webshop)
Amino acids (go to the Webshop)
NMR standards (go to the Webshop)
Biomasses (go to the Webshop)
These compounds are produced with a biotechnological approach using microorganisms such as bacteria, yeast and algae in a biotechnological approach.
Stable isotopes in Mass spectrometry
Proteins are the effectors of all activities within cells. The expression of certain proteins change upon cell stimulation or stress, or when cells differentiate or turn into a disease state. The measurement of protein expression levels as well as the characterization of their post-translational modifications gives information about their cellular state. The goal of quantitative proteomics is to evaluate the relative expression of proteins between two cellular samples being compared.
Mass spectrometry (MS) is used for the quantification and identification of molecules. For the accurate quantification of proteins, the stable isotope labelling of amino acids in cell culture (SILAC) is a versatile tool. For this purpose, reference molecules differing from the sample molecule with regards to isotopic labelling (2H, 13C, 15N) are necessary. Silantes offers methods for SILAC (Stable Isotope Labelling with Amino acids in Cell culture) and SILAM (Stable Isotope Metabolic Labelling in Mammals) to produce these reference molecules, such as
SILAC kits for in-vitro labelling (go to the Webshop)
SILAC mouse, fly and worm diets and tissues for in-vivo labelling (go to the Webshop)
SILAM mouse, fly and worm diets and tissues for in-vivo labelling (go to the Webshop)
